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The use of molecular technology provides a quick and reliable identification of sex types in plantlets growing in seedbeds.
Random amplified polymorphic DNA (RAPD) markers were used to determine the sex types of Colombian cultivars of dioecious papaya genotypes.
The sequence was found to be 1120 bp in length and based on the sequence information, a pair of sequence tagged sites primers was developed that amplified a single ~800 bp band, consistently only in all the male populations while no amplification was seen in their female counterparts.
The marker was named as Jojoba Male Sex Marker which was further validated on 330 female plants from 22 genotypes and 255 male plants from 17 genotypes. Although carefully collected, accuracy cannot be guaranteed. Differing provisions from the publisher's actual policy or licence agreement may be applicable.
The results indicate that this new SCAR marker will be valuable to determine the sex type of papaya plants.
ABSTRACT: Sex determination in plants is controlled by complex endogenous genetic programs and responses to environmental cues.
Surprisingly, the presence of well defined sex chromosomes was found in only few species.
Using conventional techniques mainly cytogenetics, sex chromosomes in some dioecious plants have been identified and characterized.
Sequencing these fragments revealed high homology, with several point mutations, four deletions in female and one in the male sequences.