Elucidating the secretion proteome of human Best site for adult sex chat with strangers
The differences between salivary proteomes may be important with respect to specific oral functions.Major efforts in salivary research have been made to characterize the salivary proteins and peptides present in parotid, submandibular, and sublingual glands and whole saliva, both by classic biochemical methods (Keller , 2005).Peptides were extracted by multiple wash and dehydration/hydration steps as described previously (Shevchenko , p H 8.0, tryptic digestion was carried out for 16 hrs at 37°C, after the addition of 2% (w/w) sequencing-grade trypsin (Promega, Madison, WI, USA).
MS/MS spectral data were searched against human protein databases (Swiss Prot and Tr EMBL, Swiss Institute of Bioinformatics, Geneva, Switzerland, by SEQUEST (Bioworks Browser 3.2, Thermo-Finnigan, San Jose, CA, USA).Recent research efforts in oral biology have resulted in elucidation of the proteomes of major human salivary secretions and whole saliva.One might hypothesize that the proteome of minor gland secretions may show significantly different characteristics when compared with the proteomes of parotid or submandibular/sublingual secretions.The total protein concentration was measured by the bicinchoninic acid (BCA) assay (Pierce Chemical, Co., Rockford, IL, USA) and bovine serum albumin protein standard.
The mean protein concentration of the labial minor salivary gland secretion pool was 1.2 mg/m L.
Salivary flow was stimulated by the application of 5% citric acid solution to the lateral border of the tongue by means of a cotton applicator.